Feline, canine and equine serum amyloid A (SAA) is a marker of inflammation
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چکیده
We offer eight well-characterized anti-SAA MAbs for the detection of feline, canine and equine SAA. These MAbs were selected from a pool of over forty MAbs that were raised against human SAA (SAA19, SAA21, SAA11), canine SAA (VSA34, VSA31, VSA38), or synthetic peptides derived from the region 79-104 a.a.r. of canine SAA (VSA2, VSA43). Isotypes and specificities of the antibodies are shown in Table 1. Six MAbs recognize feline SAA in direct ELISA and Western Blotting. Meanwhile, all MAbs recognize canine and equine SAA in direct ELISA and Western Blotting. Development of a sandwich immunoassay for feline SAA For sandwich immunoassay development for the measurement of SAA in cat serum samples, the following MAb combinations are recommended: SAA19–VSA34 and SAA21–VSA34 (capture-detection). A dilution curve of recombinant feline SAA for the MAb combination SAA19–VSA34 is provided in Figure 1. Feline, canine and equine serum amyloid A (SAA) is a marker of inflammation Product Information
منابع مشابه
Evaluation of an automated assay based on monoclonal anti-human serum amyloid A (SAA) antibodies for measurement of canine, feline, and equine SAA.
Major acute phase proteins (APPs) have proven diagnostically useful in dogs, cats and horses with routine use facilitated by commercially available automated heterologous assays. An automated assay applicable across all three species would highly facilitate further dissemination of routine use, and the aim of this study was to validate an automated latex agglutination turbidimetric immunoassay ...
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